Analysis of Sedimentation Equilibrium Distributions Reflecting Nonideal Macromolecular Associations

Analysis of sedimentation equilibrium distributions reflecting nonideal macromolecular associations.

A rigorous statistical-mechanical approach is adopted to derive general quantitative expressions that allow for the effects of thermodynamic nonideality in equilibrium measurements reflecting interaction between dissimilar macromolecular reactants. An analytical procedure based on these expressions is then formulated for obtaining global estimates of equilibrium constants and the corresponding ...

Macromolecular characterization by sedimentation equilibrium in the preparative ultracentrifuge.

Macromolecular size-and-shape distributions by sedimentation velocity analytical ultracentrifugation.

Sedimentation velocity analytical ultracentrifugation is an important tool in the characterization of macromolecules and nanoparticles in solution. The sedimentation coefficient distribution c(s) of Lamm equation solutions is based on the approximation of a single, weight-average frictional coefficient of all particles, determined from the experimental data, which scales the diffusion coefficie...

Tracer sedimentation equilibrium: a powerful tool for the quantitative characterization of macromolecular self- and hetero-associations in solution.

During the last two decades the measurement and analysis of sedimentation equilibrium has been used increasingly as a method for characterizing intermolecular interactions in solution. More recently, a variant of this technique has been developed, in which a trace amount of a single solute component is labelled so that the concentration gradient of that component at sedimentation equilibrium ma...

Computer simulation of sedimentation equilibrium distributions for systems involving heterogeneous associations. The interaction of N-acetylgucosamine with lysozyme.

Two methods are presented for computing the concentration of a species at sedimentation equilibrium as a function of radial distance, for systems wherein the species is involved in a set of chemical equilibria. The treatments are exemplified with a system involving a polymerizing acceptor and a series of acceptor-ligand complexes. It is shown that analytical expressions are available for the co...